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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/09/2023 |
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Actualizado : |
22/09/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BERAIS-RUBIO, A.; MOREL-REVETRIA, M.; FILIPPI, C.V.; REYNO, R.; MONZA, J. |
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Afiliación : |
ANDRÉS BERAIS-RUBIO, Departamento de Biología Vegetal, Laboratorio de Bioquímica, Universidad de la República, Av. Garzón 809, Montevideo PC 12.900, Uruguay; MARÍA MOREL-REVETRIA, Laboratorio de Microbiología de Suelos (LMS), Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo PC 11.400, Uruguay; CARLA VALERIA FILIPPI, Departamento de Biología Vegetal, Laboratorio de Bioquímica, Universidad de la República, Av. Garzón 809, Montevideo PC 12.900, Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE MONZA, Departamento de Biología Vegetal, Laboratorio de Bioquímica, Universidad de la República, Av. Garzón 809, Montevideo PC 12.900, Uruguay. |
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Título : |
Ensifer meliloti elite strain U143 used as alfalfa inoculant in Uruguay: Characterization and draft genome sequence. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
The Microbe. 2023, Volume 1, article 100008. https://doi.org/10.1016/j.microb.2023.100008 -- OPEN ACCESS. |
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ISSN : |
2950-1946 |
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DOI : |
10.1016/j.microb.2023.100008 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 23 May 2023, Revised 14 August 2023, Accepted 12 September 2023, Available online 13 September 2023, Version of Record 19 September 2023. -- Correspondence: Jorge Monza, mail: jmonza@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). -- FUNDING: This work was financially supported by Instituto Nacional de
Investigación Agropecuaria (INIA) L-1, Grant Comisión Académica de Postgrado, Universidad de la República, Programa de Desarrollo de
Ciencias Básicas (PEDECIBA) and Sistema Nacional de Investigadores (SNI). |
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Contenido : |
ABSTRACT.- Ensifer (syn. Sinorhizobium) meliloti U143 is an effective nitrogen-fixing strain isolated from Uruguayan soils. For decades, this strain has been used as an inoculant for different alfalfa cultivars. Here we report for the first time a characterization of the U143 elite strain that includes the preliminary genomic sequence, its annotation, and physiological parameters related to its symbiotic efficiency and nitrate respiration capacity. Through Illumina sequencing, the genome of the U143 strain was sequenced. The genome length was 6,801,966 bp, and it contained two megaplasmids, an average GC content of 62.15 %, and 6522 protein-coding sequences. In the symbiotic plasmid, we identified nap, nir, nor, and nos sequences that explain the ability of the U143 strain to respire NO3- in free-living and microaerobic conditions. Field assays performed in two locations for two years showed that alfalfa inoculated with the U143 strain produced 41 % more total shoot dry matter than the non-inoculated control, and between 61.3 % and 66.5 % of shoot N in alfalfa inoculated with strain U143 derived from nitrogen fixation. © 2023 The Authors. Published by Elsevier Ltd |
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Palabras claves : |
Draft genome; Elite strain; Inoculant. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
https://www.sciencedirect.com/science/article/pii/S2950194623000080/pdf
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Marc : |
LEADER 02580naa a2200241 a 4500
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022 $a2950-1946
024 7 $a10.1016/j.microb.2023.100008$2DOI
100 1 $aBERAIS-RUBIO, A.
245 $aEnsifer meliloti elite strain U143 used as alfalfa inoculant in Uruguay$bCharacterization and draft genome sequence.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 23 May 2023, Revised 14 August 2023, Accepted 12 September 2023, Available online 13 September 2023, Version of Record 19 September 2023. -- Correspondence: Jorge Monza, mail: jmonza@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). -- FUNDING: This work was financially supported by Instituto Nacional de Investigación Agropecuaria (INIA) L-1, Grant Comisión Académica de Postgrado, Universidad de la República, Programa de Desarrollo de Ciencias Básicas (PEDECIBA) and Sistema Nacional de Investigadores (SNI).
520 $aABSTRACT.- Ensifer (syn. Sinorhizobium) meliloti U143 is an effective nitrogen-fixing strain isolated from Uruguayan soils. For decades, this strain has been used as an inoculant for different alfalfa cultivars. Here we report for the first time a characterization of the U143 elite strain that includes the preliminary genomic sequence, its annotation, and physiological parameters related to its symbiotic efficiency and nitrate respiration capacity. Through Illumina sequencing, the genome of the U143 strain was sequenced. The genome length was 6,801,966 bp, and it contained two megaplasmids, an average GC content of 62.15 %, and 6522 protein-coding sequences. In the symbiotic plasmid, we identified nap, nir, nor, and nos sequences that explain the ability of the U143 strain to respire NO3- in free-living and microaerobic conditions. Field assays performed in two locations for two years showed that alfalfa inoculated with the U143 strain produced 41 % more total shoot dry matter than the non-inoculated control, and between 61.3 % and 66.5 % of shoot N in alfalfa inoculated with strain U143 derived from nitrogen fixation. © 2023 The Authors. Published by Elsevier Ltd
653 $aDraft genome
653 $aElite strain
653 $aInoculant
700 1 $aMOREL-REVETRIA, M.
700 1 $aFILIPPI, C.V.
700 1 $aREYNO, R.
700 1 $aMONZA, J.
773 $tThe Microbe. 2023, Volume 1, article 100008. https://doi.org/10.1016/j.microb.2023.100008 -- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
12/03/2018 |
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Tipo de producción científica : |
Documentos |
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Autor : |
BUENAHORA, J.; PINTOS, J. |
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Afiliación : |
JOSE HERMES BUENAHORA ACOSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ PINTOS. |
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Título : |
Control químico del minador de cítricos (phyllocnistis citrella Stainton) Tracer vs. Vertimek: evolución de algunos enemigos naturales en las parcelas de estudio durante el período de tratamiento. |
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Fecha de publicación : |
2001 |
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Fuente / Imprenta : |
ln: INIA Salto Grande. Avances anuales de investigación en cítricos: poscosecha, protección vegetal. Salto (Uruguay): INIA, 2001. |
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Páginas : |
p. 60-69 |
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Serie : |
(INIA Serie Actividades de Difusión ; 266) |
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Idioma : |
Español |
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Thesagro : |
CITRUS; CONTROL QUIMICO; PHYLLOCNISTIS CITRELLA. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/8905/1/Control-quimico-del-minador-de-los-citricos.pdf
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Marc : |
LEADER 00745naa a2200181 a 4500
001 1007131
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008 2001 bl uuuu u00u1 u #d
100 1 $aBUENAHORA, J.
245 $aControl químico del minador de cítricos (phyllocnistis citrella Stainton) Tracer vs. Vertimek$bevolución de algunos enemigos naturales en las parcelas de estudio durante el período de tratamiento.
260 $c2001
300 $ap. 60-69
490 $a(INIA Serie Actividades de Difusión ; 266)
650 $aCITRUS
650 $aCONTROL QUIMICO
650 $aPHYLLOCNISTIS CITRELLA
700 1 $aPINTOS, J.
773 $tln: INIA Salto Grande. Avances anuales de investigación en cítricos: poscosecha, protección vegetal. Salto (Uruguay): INIA, 2001.
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